研究発表
研究論文/publications
■研究論文/publications
1. Sun, L. Nakamae, N., and Ichikawa, N. (2015) The region from
phenylalanine-28 to lysine-50 of a yeast mitochondrial ATPase inhibitor (IF1)
forms an α-helix in solution. J. Bioenerg.
Biomembr. 47, 457-465 (doi: 10.1007/s10863-015-9627-7)
[Full text link]
2. Ando, C. and Ichikawa, N. (2008) Glutamic acid in the inhibitory site of
mitochondrial ATPase inhibitor, IF1, participates in pH sensing in both mammals
and yeast. J. Biochem. 144, 547-553 (doi:
10.1093/jb/mvn100)
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3. Ichikawa, N., Ando, C., and Fumino, M. (2006) Caenorhabditis elegans MAI-1
protein, which is similar to mitochondrial ATPase inhibitor (IF1), can inhibit
yeast F0F1-ATPase but cannot be transported to yeast
mitochondria. J. Bioenerg. Biomembr. 38, 93-99
(doi: 10.1007/s10863-006-9009-2)
4. Ichikawa, N., Chisuwa, N., Tanase, M., and Nakamura, M. (2005) Mitochondrial ATP synthase residue βArginine-408,
which interacts with the inhibitory site of regulatory protein IF1, is
essential for the function of the enzyme. J. Biochem. 138, 201-207
(doi: 10.1093/jb/mvi116)
5. Ichikawa N. and Mizuno, M. (2004) Functional
expression of hexahistidine-tagged β-subunit of yeast F1-ATPase and isolation of the enzyme by immobilized metal
affinity chromatography. Protein Expr. Purif. 37, 97-101
(doi: 10.1016/j.pep.2004.06.002)
6. Ichikawa, N. and Ogura, C. (2003) Overexpression,
purification, and characterization of human and bovine mitochondrial ATPase
inhibitors: comparison of the properties of mammalian and yeast ATPase
inhibitors. J. Bioenerg. Biomembr. 35, 399-407
7. Ichikawa, N., Nakabayashi, K., and Hashimoto, T. (2002) A yeast mitochondrial ATPase inhibitor interacts with three proteins
that are easy to dissociate from the mitochondrial inner membrane. J.
Biochem. 132, 649-654
8. Ichikawa, N., Karaki, A., Kawabata, M., Ushida, S., Mizushima, M., and
Hashimoto, T. (2001) The region from phenylalanine-17
to phenylalanine-28 of a yeast mitochondrial ATPase inhibitor is essential for
its ATPase inhibitory activity. J. Biochem. 130, 687-693
9. Ichikawa, N, Fujisaka, R, and Kuribayashi, R. (2000) Requirement for
lysine-19 of the yeast mitochondrial ATPase inhibitor for the stability of the
inactivated inhibitor-F1Fo complex at higher pH. Biosci. Biotechnol.
Biochem. 64, 89-95
10. Ichikawa, N., Ushida, S., Kawabata, M. and Masazumi, Y. (1999) Nucleotide
Sequence of cDNA Coding the Mitochondrial Precursor Protein of the ATPase
Inhibitor from Humans. Biosci. Biotechnol. Biochem. 63, 2225-2227
11. Ichikawa, N., Fukuda, M., Hashimoto, T., and Tagawa, K. (1998) The
carboxyl-terminal region of the yeast ATPase inhibitor is indispensable for the
stability of the protein in mitochondria. J. Biochem. 124, 886-891
12. Ichikawa, N., Yoshida, Y., Hashimoto, T., and Tagawa, K. (1996) An
intrinsic ATPase inhibitor binds near the active site of yeast mitochondrial F1-ATPase. J. Biochem. 119, 193-199
13. Ichikawa, N., Yoshida, Y., Hashimoto, T., Ogasawara, N., Yoshikawa, H.,
Imamoto, F., and Tagawa, K. (1990) Activation of ATP hydrolysis by an uncoupler
in mutant mitochondria lacking an intrinsic ATPase inhibitor in yeast. J.
Biol. Chem. 265, 6274-6278
主な著書/Books
■主な著書/Books
1. 市川直樹、田川邦夫. 廣川タンパク質化学７ 制御タンパク質（インヒビター）「ATPaseインヒビター」 廣川書店 (2002)
学会活動/Membership in Academic Societies
■学会活動/Membership in Academic Societies
日本生化学会、他