原田敦史

harada

氏名　　：　　原田　敦史（はらだ　あつし）
部屋　　：　　B5棟 5階 5A - 02
TEL&FAX ：　072-254-9328
Email ：

学歴

東京理科大学基礎工学部材料工学科卒業(1994)
東京理科大学大学院博士前期課程修了(1996)
東京理科大学大学院博士後期課程中退(1998)
博士取得（東京大学）(2002)

職歴

日本学術振興会特別研究員（DC2）(1998)
東京大学大学院工学系研究科材料学専攻助手(1998)
東京大学大学院工学系研究科マテリアル工学専攻助手(2002)
大阪府立大学大学院工学研究科機能物質科学分野講師(2003)
大阪府立大学大学院工学研究科応用化学分野助教授(2005)
大阪府立大学大学院工学研究科応用化学分野准教授(2007)
大阪府立大学大学院工学研究科応用化学分野教授(2018)

学位

工学博士（東京大学　2002）

学位論文： "Structural Design of Novel Supramolecular Assembly from Charged Block Copolymers and Its Utilities as Functional Materials"

所属学会

高分子学会，日本バイオマテリアル学会，日本化学会，日本ＤＤＳ学会

American Chemical Society，Controlled Release Society

原著論文・総説

2019

146) M. Miyazaki, E. Yuba, H. Hayashi, A. Harada, K. Kono, "Development of pH-responsive hyaluronic acid-based antigen carriers for induction of antigen-specific cellular immune responses", ACS Biomaterials Science & Engineering, 5, 5790-5797 (2019). DOI: 10.1021/acsbiomaterials.9b01278.
145) M. Okubo, M. Miyazaki, E. Yuba, A. Harada, "Chondroitin sulfate-based pH-sensitive polymer-modified liposomes for intracellular antigen delivery and induction of cancer immunity", Bioconjugate Chemistry, 30, 1518-1529 (2019). DOI: 10.1021/acs.bioconjchem.9b00221.
144) A. Harada, K. Nomura, E. Yuba, K. Kono, "Gene expression of aspect ratio-controlled polyplexes based on the effect of multi-arm poly(ethylene glycol)", ACS Biomaterials Science & Engineering, 5, 5681-5687 (2019). DOI: 10.1021/acsbiomaterials.8b01498.
143) S. Kaviarasi, E. Yuba, A. Harada, U. M. Krishnan, "Emerging paradigms in nanotechnology for imaging and treatment of cerebral ischemia", J. Control. Release, 300, 22-45 (2019). DOI: 10.1016/j.jconrel.2019.02.031.
142) S. Kaviarasi, K. S. S. Devi, P. Vinoth, V. Sridharan, E. Yuba, A. Harada, U. M. Krishnan, “Synthesis, characterization, and biomedical applications of an alkylated quercetin–gadolinium complex”, ACS Biomaterials Science & Engineering, 5, 1215-1227 (2019). DOI: 10.1021/acsbiomaterials.8b01254.
141) R. Teranishi, T. Matsuda, E. Yuba, K. Kono, A. Harada, "Sonodynamic therapeutic effects of sonosensitizers with different intracellular distribution delivered by hollow nanocapsules exhibiting cytosol specific release", Macromolecular Bioscience, 19, e1800365 (2019). DOI: 10.1002/mabi.201800365.
140) S. Kuroyanagi, N. Shimada, S. Fujii, T. Furuta, A. Harada, K. Sakurai, A. Maruyama, “Highly ordered polypeptide with UCST phase-separation behavior”, Journal of the American Chemical Society, 141, 1261-1268 (2019). DOI: 10.1021/jacs.8b10168.

2018

140) T. Hashimoto, E. Yuba, A. Harada, K. Kono,“Photothermal-chemotherapy nanohybrid preparation by complexation of gold nanorods with polyamidoamine dendrimers having poly(ethylene glycol) and hydrophobic chains”, J. Mater. Chem. B, , accepted. DOI:
139) 山本聡, 古川和樹, 小野雅文, 弓場英司, 原田敦史, 河野健司，「TiO₂ナノ粒子内包ポリイオンコンプレックスミセルによる疎水性プロドラッグの可溶化と超音波照射効果」,高分子論文集, < 75, 42-47 (2018). DOI: 10.1295/koron.2017-0063.
138) M. Miyazaki, E. Yuba, H. Hayashi, A. Harada, K. Kono, “Hyaluronic acid-based pH-sensitive polymer-modified liposomes for cell-specific intracellular drug delivery systems”, Bioconjugate Chem., 29, 44-55 (2018). DOI: 10.1021/acs.bioconjchem.7b00551.
137) E. Yuba, T. Osaki, M. Ono, S. Park, A. Harada, M. Yamashita, K. Azuma, T. Tsuka, N. Ito, T. Imagawa, Y. Okamoto, “Bleomycin-loaded pH-sensitive polymer-lipid-incorporated liposomes for cancer chemotherapy”, Polymers, 10, 74 (2018). DOI: 10.3390/polym10010074. [Open Access]
136) A. Harada, K. Kataoka, “Polyion complex micelle (PIC micelle) formation from double-hydrophilic block copolymers composed of charged and non-charged segments in aqueous medium”, Polymer Journal, 50, 95-100 (2018). DOI: 10.1038/pj.2017.67. [Free Access]
135) 弓場英司，原田敦史，「DDS応用を指向した刺激応答性リポソームの設計」,ファルマシア 54, 11-15 (2018). DOI: 10.14894/faruawpsj.54.1_11.
134) N. Yamazaki, S. Yamakawa, T. Sugimoto, Y. Yoshizaki, R. Teranishi, T. Hayashi, A. Kotaka, C. Shinde, T. Kumei, Y. Sumida, T. Shimizu, Y. Ohashi, E. Yuba, A. Harada, K. Kono, “Carboxylated phytosterol derivative-introduced liposomes for skin environment-responsive transdermal drug delivery system”, J. Liposome Res., 28, 275-284 (2018). DOI: 10.1080/08982104.2017.1369995.

2017

133) S. Yamamoto, M. Ono, E. Yuba, A. Harada, “In vitro sonodynamic therapeutic effect of polyion complex micelles incorporating titanium dioxide nanoparticles”, Nanomaterials, 7, 268 (2017). DOI: 10.3390/nano7090268. [Open Access]
132) E. Yuba, S. Uesugi, M. Miyazaki, Y. Kado, A. Harada, K. Kono, “Development of pH-sensitive dextran derivatives with strong adjuvant function and their application to antigen delivery”, Membranes, 7, 41 (2017). DOI: 10.3390/membranes7030041. [Open Access]
131) Y. Yoshizaki, E. Yuba, N. Sakaguchi, K. Koiwai, A. Harada, K. Kono, “pH-sensitive polymer-modified liposome-based immunity-inducing system: effects of inclusion of cationic lipid and CpG-DNA”, Biomaterials, 141, 272-283 (2017). DOI: 10.1016/j.biomaterials.2017.07.001.
130) Y. Inaba, K. Watanabe, M. Kitamatsu, E. Nakata, A. Harada, T. Ohtsuki, “Ultrasound-dependent cytoplasmic internalization of a peptide-sonosensitizer conjugate”, Bioorganic & Medicinal Chemistry, 25, 4212-4217 (2017). DOI: 10.1016/j.bmc.2017.06.024.
129)　A. Harada, R. Teranishi, E. Yuba, K. Kono, “Effect of the side chain spacer structure on the pH-responsive properties of polycarboxylates”, Journal of Biomaterials Science: Polymer Edition, 28, 1025-1035 (2017). DOI: 10.1080/09205063.2017.1324551.
128) E. Yuba, S. Uesugi, Y. Yoshizaki, A. Harada, K. Kono, “Potentiation of cancer immunity-inducing effect by pH-sensitive polysaccharide-modified liposomes with combination of TGF-β type I receptor inhibitor-embedded liposomes”, Med. Res. Arch., 5, (2017). DOI: 10.18103/mra.v5i5.1243. [Open Access]
127) T．Sugimoto, N. Yamazaki, T. Hayashi, E. Yuba, A. Harada, A. Kotaka, C. Shinde, T. Kumei, Y. Sumida, M. Fukushima, Y. Munekata, K. Maruyama, K. Kono, “Preparation of dual-stimuli-responsive liposomes using methacrylate-based copolymers with pH and temperature sensitivities for precisely controlled release”, Colloids Surf. B: Biointerfaces, 155, 449-458 (2017). DOI: 10.1016/j.colsurfb.2017.04.043.
126) N. Yamazaki, T. Sugimoto, M. Fukushima, R. Teranishi, A. Kotaka, C. Shinde, T. Kumei, Y. Sumida, Y. Munekata, K. Maruyama, E. Yuba, A. Harada, K. Kono, “Dual-stimuli responsive liposomes using pH- and temperature-sensitive polymers for controlled transdermal delivery”, Polymer Chemistry, 8, 1507-1518 (2017). DOI: 10.1039/c6py01754a.
125) R. Teranishi, R. Matsuki, E. Yuba, A. Harada, K. Kono, “Doxorubicin delivery using pH and redox dual-responsive hollow nanocapsules with a cationic electrostatic barrier”, Pharmaceutics, 9, 4 (2017). DOI: 10.3390/pharmaceutics9010004. [Open Access]
124) E. Yuba, A. Yamaguchi, Y. Yoshizaki, A. Harada, K. Kono, “Bioactive polysaccharide-based pH-sensitive polymers for cytoplasmic delivery of antigen and activation of antigen-specific immunity”, Biomaterials, 120, 32-45 (2017). DOI: 10.1016/j.biomaterials.2016.12.021.

2016

123) Y. Yoshizaki, E. Yuba, T. Komatsu, K. Udaka, A. Harada, K. Kono, “Improvement of peptide-based tumor immunotherapy using pH-sensitive fusogenic polymer-modified liposomes”, Molecules, 21, 1284 (2016). DOI:10.3390/molecules21101284. [Open Access]
122)弓場英司，原田敦史, 河野健司, 「免疫治療のためのDDS最前線」,高分子, 65, 435-440 (2016).
121) Y. Xu, M. Shinoyama, A. Harada, “Soft matter-regulated active nanovalves locally self-assembled in femtoliter nanofluidic channels”, Advanced Materials, 28, 2209-2216 (2016). DOI:10.1002/adma.201505132.

2015

120) K. Kono, M. Takashima, E. Yuba, A. Harada, Y. Hiramatsu, H. Kitagawa, T. Otani, K. Maruyama, S. Aoshima, “Multifunctional liposomes having target specificity, temperature-triggered release, and near-infrared fluorescence imaging for tumor-specific chemotherapy”, J. Control. Release, 216, 69-77 (2015). DOI: 10.1016/j.jconrel.2015.08.005.
119) S. Yamamoto, E. Yuba, A. Harada, K. Kono, "Effective condensation of multivalent anions into polyion complex micelles prepared from TiO₂ nanoparticles and polyallylamine bearing poly(ethylene glycol) grafts", Langmuir, 31, 8583-8588 (2015). DOI: 10.1021/acs.langmuir.5b01883.
118) E. Yuba, Y. Kanda, Y. Yoshizaki, R. Teranishi, A. Harada, K. Sugiura, T. Izawa, J. Yamate, N. Sakaguchi, K. Koiwai, K. Kono, “pH-Sensitive polymer-liposome-based antigen delivery systems potentiated with interferon-γ gene lipoplex for efficient cancer immunotherapy”, Biomaterials, 67, 214-224 (2015). DOI: 10.1016/j.biomaterials.2015.07.031.
117) T. Doura, M. Yamada, R. Teranishi, Y. Yamamoto, T. Sugimoto, E. Yuba, A. Harada, K. Kono, "PAMAM dendron lipid assemblies that undergo structural transition in response to weakly acidic pH and their cytoplasmic delivery capability", Langmuir, 31, 5105-5114 (2015). DOI: 10.1021/acs.langmuir.5b00183.
116) R. Aono, K. Nomura, E. Yuba, A. Harada, K. Kono , "Gene expression of ternary complexes through the compaction of nanofiber-polyplexes by mixing with Lipofectamine", Biomaterials Science, 3, 764-770 (2015). DOI: 10.1039/C5BM00081E.
115)　X. Li, Y. Watanabe, E. Yuba, A. Harada, T. Haino, K. Kono, "Facile construction of well-defined fullerene-dendrimer supramolecular nanocomposites for bioapplications", Chem. Commun., 51, 2851-2854 (2015). DOI: 10.1039/C4CC09082A.

2014

114)　K. Kono, T. Kaiden, E. Yuba, Y. Sakanishi, A. Harada, "Synthesis of oligoethylene glycol-modified hyperbranched polyglycidols for dual sensitization of liposomes to pH and temperature", J. Taiwan Institute of Chemical Engineers, 45, 3054-3061 (2014). DOI：10.1016/j.jtice.2014.06.011.
113)　Y. Yoshizaki, E. Yuba, N. Sakaguchi, K. Koiwai, A. Harada, K. Kono, “Potentiation of pH-sensitive polymer-modified liposomes with cationic lipid inclusion as antigen delivery carriers for cancer immunotherapy”, Biomaterials, 35, 8186-8196 (2014). DOI:10.1016/j.biomaterials.2014.05.077.
112)　K. Kono, K. Takeda, X. Li, E. Yuba, A. Harada, T. Ozaki, S. Mori, "Dually functionalized dendrimers by temperature-sensitive surface modification and gold nanoparticle loading for biomedical application", RSC Adv., 4, 27811-27819 (2014). DOI: 10.1039/c4ra03947e.
111)　X. Li, K. Takeda, E. Yuba, A. Harada, K. Kono, “Preparation of PEG-modified PAMAM dendrimers having gold nanorod core and their application to photothermal therapy”, J. Mater. Chem. B, 2, 4167-4176 (2014). DOI:10.1039/C4TB00132J.
110)　X. Li, M. Takashima, E. Yuba, A. Harada, K. Kono, “PEGylated PAMAM dendrimer-doxorubicin conjugate-hybridized gold nanorod for combined photothermal-chemotherapy”, Biomaterials, 35, 6576-6584 (2014). DOI:10.1016/j.biomaterials.2014.04.043.
109)　R. Aono, E. Yuba, A. Harada, K. Kono, "Nanofiber polyplex formation based on the morphology elongation by the intra-polyplex PEG crowding effect", ACS Macro Lett., 3, 333-336 (2014). DOI: 10.1021/mz500072k.
108)　E. Yuba, N. Tajima, Y. Yoshizaki, A. Harada, H. Hayashi, K. Kono, "Dextran derivative-based pH-sensitive liposomes for cancer immunotherapy", Biomaterials, 35, 3091-3101 (2014). DOI:10.1016/j.biomaterials.2013.12.024.

2013

107)　C. Kojima, E. Nishisaka, T. Suehiro, K. Watanabe, A. Harada, T. Goto, Y. Magata, K. Kono, "The synthesis and evaluation of polymer prodrug/collagen hybrid gels for delivery into metastatic cancer cells", Nanomedicine-Nanotechnology, Biology and Medicine, 9, 767-775 (2013). DOI:10.1016/j.nano.2013.01.004.
106) A. Harada, R. Matsuki, S. Ichimura, E. Yuba, K. Kono, "Intracellular environment-responsive stabilization of polymer vesicles formed from head-tail type polycations composed of polyamidoamine dendron and poly (L-lysine)", Molecules, 18, 12168-12179 (2013). DOI:10.3390/molecules181012168. [Open Access]
105)　C. Kojima, H. Kawabata, A. Harada, H. Horinaka, K. Kono, "Design of a novel drug carrier with photo-responsive properties: drug-encapsulated and alkanethiol-modified gold nanoparticle-loaded PEGylated dendrimer", Chem. Lett., 42, 612-614 (2013). DOI:10.1246/cl.130079.
104) E. Yuba, Y. Kono, A. Harada, S. Yokoyama, M. Arai, K. Kubo, K. Kono, "The application of pH-sensitive polymer-lipids to antigen delivery for cancer immunotherapy", Biomaterials, 34, 5711-5721 (2013). DOI:10.1016/j.biomaterials.2013.04.007.
103)　X. Li, Y. Haba, K. Ochi, E. Yuba, A. Harada, K. Kono, "PAMAM dendrimers with oxyethylene unit-enriched surface as biocompatible temperature-sensitive dendrimers", Bioconjugate Chem., 24, 282-290 (2013). DOI:10.1021/bc300190v.
102)　E. Yuba, A. Harada, Y. Sakanishi, S. Watarai, K. Kono, "A liposome-based antigen delivery system using pH-sensitive fusogenic polymers for cancer immunotherapy", Biomaterials, 34, 3042-3052 (2013). DOI:10.1016/j.biomaterials.2012.12.031.
101) C. Kojima, T. Suehiro, K. Watanabe, M. Ogawa, A. Fukuhara, E. Nishisaka, A. Harada, K. Kono, T. Inui, Y. Magata, "Doxorubicin-conjugated dendrimer/collagen hybrid gels for metastasis-associated drug delivery system”, Acta Biomaterialia, 9, 5673-5680 (2013). DOI:10.1016/j.actbio.2012.11.013.
100)　A. Harada, M. Ono, E. Yuba, K. Kono, "Titanium dioxide nanoparticles-entrapped polyion complex micelles generate singlet oxygen in the cells by ultrasound irradiation for sonodynamic therapy", Biomaterials Science, 1, 65-73 (2013). DOI: 10.1039/C2BM00066K.

2012

99)　E. Yuba, Y. Nakajima, K. Tsukamoto, S. Iwashita, C. Kojima, A. Harada, K. Kono, "Effect of unsaturated alkyl chains on transfection activity of poly(amidoamine) dendron-bearing lipids", J. Control. Release, 160, 552-560 (2012). DOI:10.1016/j.jconrel.2012.04.002.
98)　K. Kono, R. Ikeda, K. Tsukamoto, E. Yuba, C. Kojima, A. Harada, "Polyamidoamine dendron-bearing lipids as a non-viral vector: influence of dendron generation", Bioconjugate Chem., 23, 871-879 (2012). DOI:10.1021/bc200368b.

2011

97)　T. Kaiden, E. Yuba, A. Harada, Y. Sakanishi, K. Kono, "Dual signal-responsive liposomes for temperature-controlled cytoplasmic delivery", Bioconjugate Chem., 22(10), 1909-1915 (2011). DOI:10.1021/bc2000353.
96)　A. Harada, T. Kato, A. Kawamura, C. Kojima, K. Kono, "Effect of enzyme modification by well-defined multi-armed poly(ethylene glycol) synthesized using polyamidoamine dendron", J. Biomaterials Science: Polymer Edition, 22(12), 1551-1591 (2011). DOI:10.1163/092050610x514557.
95)　K. Kono, E. Murakami, Y. Hiranaka, E. Yuba, C. Kojima, A. Harada, K. Sakurai, "Thermosensitive molecular assemblies from PAMAM dendron-based lipids", Angew. Chem. Int. Ed., 50, 6332-6336 (2011). DOI:10.1002/anie.201101007.
94)　A. Harada, S. Ichimura, E. Yuba, K. Kono, "Hollow nanocapsules prepared through stabilization of polymer vesicles formed from head-tail type polycations by introducing cross-linkages", Soft Matter, 7(10), 4629-4635 (2011). DOI:10.1039/c1sm05075c.
93)　K. Kono, S. Nakashima, D. Kokuryo, I. Aoki, H. Shimomoto, S. Aoshima, K. Maruyama, E. Yuba, C. Kojima, A. Harada, Y. Ishizaka, "Multifunctional liposomes having temperature-triggered release and magnetic resonance imaging for tumor-specific chemotherapy", Biomaterials, 32, 1387-1395 (2011). DOI:10.1016/j.biomaterials.2010.10.050.
92)　E. Yuba, A. Harada, Y. Sakanishi, K. Kono, "Carboxylated hyperbranched poly(glycidol)s for preparation of pH-sensitive liposomes", J. Control. Release, 149, 72-80 (2011). DOI:10.1016/j.jconrel.2010.03.001.

2010

91)　A. Harada, Y. Kimura, K. Kono, "Cationic polymers with inhibition ability of DNA condensation elevate gene expression", ChemBioChem, 11, 1985-1988 (2010). DOI:10.1002/cbic.201000394.
90)　C. Kojima, Y. Umeda, A. Harada, K. Kono, "Preparation of near-infrared light absorbing gold nanoparticles using polyethylene glycol-attached dendrimers", Colloids Surf. B: Biointerfaces, 81, 648-651 (2010). DOI:10.1016/j.colsurfb.2010.07.060.
89) C. Kojima, K. Yoshimura, A. Harada, Y. Sakanishi, K. Kono, "Temperature-sensitive hyperbranched poly(glycidol)s with oligo(ethylene glycol) monoethers", J. Polym. Sci. Part A: Polym. Chem., 48, 4047-4054 (2010). DOI:10.1002/pola.24190.
88)　Y. Umeda, C. Kojima, A. Harada, H. Horinaka, K. Kono, "PEG-attached PAMAM dendrimers encapsulating gold nanoparticles: growing gold nanoparticles in the dendrimers for improvement of their photothermal properties", Bioconjugate Chem., 21, 1559-1564 (2010). DOI:10.1021/bc1001399.
87)　K. Kono, T. Ozawa, T. Yoshida, F. Ozaki, Y. Ishizaka, K. Maruyama, C. Kojima, A. Harada, S. Aoshima, "Highly temperature-sensitive liposomes based on a thermosensitive block copolymer for tumor-specific chemotherapy", Biomaterials, 31, 7096-7105 (2010). DOI:10.1016/j.biomaterials.2010.05.045.
86)　C. Kojima, Y. Umeda, M. Ogawa, A. Harada, Y. Magata, K. Kono, "X-ray computed tomography contrast agents prepared by seeded growth of gold nanoparticle in PEGylated dendrimer", Nanotechnology, 21, 245104 (2010). DOI:10.1088/0957_4484/21/24/245104.
85)　A. Harada, Y. Kimura, C. Kojima, K. Kono, "Effective tolerance to serum proteins of head-tail type polycation vectors by PEGylation at the periphery of the head block", Biomacromolecules, 11, 1036-1042 (2010). DOI:10.1021/bm1000108.
84)　T. Suehiro, C. Kojima, S. Tsumura, A. Harada, K. Kono, "Higher order structure of short collagen model peptides attached to dendrimers and linear polymers", Biopolymers, 93, 640-648 (2010). DOI:10.1002/bip.21413.
83)　A. Harada, A. Yuzawa, T. Kato, C. Kojima, K. Kono, "Effect of pH and generation of dendron on single-step synthesis of gold nanoparticles using PEGylated polyamidoamine dendron in aqueous medium", J. Polym. Sci. Part A: Polym. Chem., 48, 1391-1398 (2010). DOI:10.1002/pola.23901.
82)　E. Yuba, C. Kojima, A. Harada, Tana, S. Watarai, K. Kono, "pH-Sensitive fusogenic polymer-modified liposomes as a carrier of antigenic proteins for activation of cellular immunity", Biomaterials, 31, 943-951 (2010). DOI:10.1016/j.biomaterials.2009.10.006.

2009

81)　T. Takahashi, E. Yuba, C. Kojima, A. Harada, K. Kono, "Synthesis of a polyamidoamine dendron-bearing lipid having sugar moieties and its use for preparation of nonviral gene vectors", Res. Chemical Intermediates, 35, 1005-1014 (2009). DOI:10.1007/s11164_009_0083_z.
80)　A. Harada, M. Kawamura, Y. Kimura, T. Takahashi, C. Kojima, K. Kono, "Effect of head size in head-tail type polycations on their in vitro performances as non-viral gene vectors", Macromol. Biosci., 9, 605-612 (2009). DOI:10.1002/mabi.200800314.
79)　C. Kojima, S. Tsumura, A. Harada, K. Kono, "A collagen-mimic dendrimer capable of controlled release", J. Am. Chem. Soc., 131, 6052-6053 (2009). DOI:101021/ja809639c.
78)　C. Kojima, K. Yoshimura, A. Harada, Y. Sakanishi, K. Kono, "Synthesis and characterization of hyperbranched poly(glycidol) modified with pH- and temperature-sensitive groups", Bioconjugate Chem., 20, 1054-1057 (2009). DOI:10.1021/bc900016x.
77)　A. Harada, K. Nakanishi, S. Ichimura, C. Kojima, K. Kono, "Spontaneous formation of narrowly-distributed self-assembly from polyamidoamine dendron-poly(L-lysine) block copolymers through helix-coil transition of poly(L-lysine) block", J. Polym. Sci. Part A: Polym. Chem., 47, 1217-1223 (2009). DOI:10.1002/pola.23231.

2008

76)　C. Kojima, Y. Toi, A. Harada, K. Kono, "Aqueous solubilization of fullerenes using poly(amidoamine) dendrimers bearing cyclodextrin and poly(ethylene glycol)", Bioconjugate Chem., 19, 2280-2284 (2008). DOI:10.1021/bc8001503.
75)　C. Kojima, Y. Hirano, E. Yuba, A. Harada, K. Kono, "Preparation and characterization of complexes of liposomes with gold nanoparticles", Colloids Surf. B:Biointerfaces, 66, 246-252 (2008). DOI:10.1016/j.colsurfb.2008.06.022.
74) N. Sakaguchi, C. Kojima, A Harada, K. Koiwai, K. Kono, "The correlation between fusion capability and transfection activity in hybrid complexes of lipoplexes and pH-sensitive liposomes", Biomaterials, 29, 4029-4036 (2008). DOI:10.106/j.biomaterials.2008.06.006.
73) N. Sakaguchi, C. Kojima, A. Harada, K. Koiwai, N. Emi, K. Kono, "Effect of transferrin as a ligand of pH-sensitive fusogenic liposome-lipoplex hybrid complexes", Bioconjugate Chem., 19, 1588-1595 (2008). DOI:10.1021/bc800126s.
72) E. Yuba, C. Kojima, N. Sakaguchi, A. Harada, K. Koiwai, K. Kono, "Gene delivery to dendritic cells mediated by complexes of lipoplexes and pH-sensitive fusogenic polymer-modified liposomes", J. Control. Release, 130, 77-83 (2008). DOI:10.1016/j.jconrel.2008.05.007.
71)　N. Sakaguchi, C. Kojima, A. Harada, K. Kono, Preparation of pH-sensitive poly(glycidol) derivatives with varying hydrophobicities: their ability to sensitize stable liposomes to pH, Bioconjugate Chem., 2008, 19 (5), 1040-1048
70)　A. Kawamura, C. Kojima, M. Iijima, A. Harada, K. Kono, Polyion complex micelles formed from glucose oxidase and comb-type polyelectrolyte with poly(ethylene glycol) grafts, J. Polym. Sci. Part A: Polym. Chem., 2008, 46 (11), 3842-3852
69)　K. Kono, C. Kojima, N. Hayashi, E. Nishisaka, K. Kiura, S. Watarai, A. Harada, Preparation and cytotoxic activity of poly(ethylene glycol)-modified poly(amidoamine) dendrimers bearing adriamycin, 2008, Biomaterials, 29 (11), 1664-1675
68)　N. Sakaguchi, C. Kojima, A. Harada, K. Koiwai, K. Shimizu, N. Emi, K. Kono, Generation of highly potent nonviral gene vectors by complexation of lipoplexes and transferrin-bearing fusogenic polymer-modified liposomes in aqueous glucose solution, 2008, Biomaterials, 29 (9), 1262-1272

2007

67)　A. Harada, K. Johnin, A. Kawamura, K. Kono, Preparation of temperature-responsive polymer gels physically immobilizing core-shell type bioconjugates, J. Polym. Sci. Part A: Polym. Chem., 2007, 45 (24), 5942-5948
66)　A. Kawamura, A. Harada, K. Kono, K. Kataoka, Self-assembled nano-bioreactor from block ionomers with elevated and stabilized enzymatic function, Bioconjugate Chem., 2007, 18 (5), 1555-1559
65)　K. Kono, T. Miyoshi, Y. Haba, E. Murakami, C. Kojima, A. Harada, Temperature sensitivity control of alkylamide-terminated poly(amidoamine) dendrimers induced by guest molecule binding, J. Am. Chem. Soc., 2007, 129 (23), 7222-7223
64)　T. Takahashi, J. Hirose, C. Kojima, A. Harada, K. Kono, Synthesis of poly(amidoamine) dendron-bearing lipids with poly(ethylene glycol) grafts and their use for stabilization of nonviral gene vectors, Bioconjugate Chem., 2007, 18 (4), 1163-1169
63)　T. Takahashi, C. Kojima, A. Harada, K. Kono, Alkyl chain moieties of polyamidoamine dendron-bearing lipids influence their function as a nonviral gene vector, Bioconjugate Chem., 2007, 18 (4), 1349-1354
62)　Y. Haba, C. Kojima, A. Harada, T. Ura, H. Horinaka, K. Kono, Preparation of poly(ethylene glycol)-modified poly(amidoamine) dendrimers encapsulating gold nanoparticles and their heat-generating ability, Langmuir, 2007, 23 (10), 5243-5246
61)　C. Kojima, Y. Toi, A. Harada, K. Kono, Preparation of polyethylene glycol-attached dendrimers encapsulating photosensitizers for application to photodynamic therapy, Bioconjugate Chem., 2007, 18 (3), 663-670
60)　原田敦史・片岡一則，ナノバイオデバイスとして機能する高分子ミセル設計，オレオサイエンス，2007, 7 (3), 111-118
59)　A. Harada, Y. Yoshioka, A. Kawamura, C. Kojima, K. Kono, "Effect of polycarboxylate block on the amidase activity of trypsin through complexation with peg-polycarboxylate block ionomers", Macromol. Biosci., 2007, 7 (3), 339-343
58)　Y. Haba, C. Kojima, A. Harada, K. Kono, Comparison of thermosensitive properties between poly(amidoamine) dendrimers having peripheral N-isopropylamide groups and linear polymers with the same groups, Angew. Chem. Int. Ed., 2007, 46 (1-2), 234-237

2006

57)　A. Harada, K. Kataoka, Supramolecular assemblies of block copolymers in aqueous media as nanocontainers relevant to biological applications, Prog. Polym. Sci., 2006, 31 (11), 949-982
56)　A. Harada, K. Kataoka, Stimuli responsive polyion complex assemblies, MML Series 7, Smart Nano and Microparticles, K. Kono, R. Arshady (eds.), 2006, pp. 210-229, Kentus Books, London
55)　Y. Haba, C. Kojima, A. Harada, K. Kono, Control of temperature-sensitive properties of poly(amidoamine) dendrimers using peripheral modification with various alkylamide groups, Macromolecules, 2006, 39 (21), 7451-7453
54)　原田敦史，遺伝子ベクターとしてのヘッド－テイル型ポリカチオンブロック共重合体の分子設計，バイオマテリアル，2006, 24 (3), 171-175
53)　N. Sakaguchi, C. Kojima, A. Harada, K. Koiwai, K. Shimizu, N. Emi, K. Kono, Enhancement of transfection activity of lipoplexes by complexation with transferrin-bearing fusogenic polymer-modified liposomes, Int. J. Pharm., 2006, 325 (1-2), 186-190
52)　Y. Tono, C. Kojima, Y. Haba, T. Takahashi, A. Harada, S. Yagi, K. Kono, Thermosensitive properties of poly(amidoamine) dendrimers with peripheral phenylalanine residues, Langmuir, 2006, 22 (11), 4920-4922
51)　A. Harada, M. Kawamura, T. Matsuo, T. Takahashi, K. Kono, Synthesis and characterization of head-tail type polycation block copolymer as non-viral gen e vector, Bioconjugate Chem., 2006, 17 (1), 3-5

2005

50)　T. Takahashi, A. Harada, N. Emi, K. Kono, Preparation of efficient gene carriers using a polyamidoamine dendron-bearing lipid: improvement of serum resistance, Bioconjugate Chem., 2005, 16 (5), 1160-1165
49)　A. Kawamura, Y. Yoshioka, A. Harada, K. Kono, Acceleration of enzymatic reaction of trypsin through the formation of water-soluble complexes with poly(ethylene glycol)-block-poly(α, β-aspartic acid), Biomacromolecules, 2005, 6 (2), 627-631
48)　X. Yuan, A. Harada, Y. Yamasaki, K. Kataoka, Stabilization of Lysozyme-Incorporated Polyion Complex (PIC) Micelle by the ω-End-Derivatization of Poly(ethylene glycol)-Poly(α, β-aspartic acid) Block Copolymer with Various Hydrophobic Groups, Langmuir, 2005, 21 (7), 2668-2674
47)　Y. Haba, A. Harada, T. Takagishi, K. Kono, Synthesis of biocompatible dendrimers with a peripheral network formed by linking of polymerizable groups, Polymer, 2005, 46 (6), 1813-1820
46)　K. Kono, H. Akiyama, T. Takahashi, T. Takagishi, A. Harada, Transfection Activity of Polyamidoamine Dendrimers Having Hydrophobic Amino Acid Residues in the Periphery, Bioconjugate Chem., 2005, 16 (1), 208-214
45)　W.-D. Jang, N. Nishiyama, G.-D. Zhang, A. Harada, D.-L. Jiang, S. Kawauci, Y. Morimoto, M. Kikuchi, H. Koyama, T. Aida, K. Kataoka, Suparmolecular nanocarrier of anionic dendrimer porphyrins with PEGylated cationic block copolymer to enhance intracellular photodynamic efficacy, Angew. Chem. Int. Ed., 2005, 44 (3), 419-423

2004

44)　原田敦史，内核に酵素反応場を有するコア－シェル型ナノ組織体の調製とバイオナノリアクターとしての機能，高分子加工，2004, 53 (12), 12-16
43)　D. Wakebayashi, N. Nishiyama, K. Itaka, K. Miyata, Y. Yamasaki, A. Harada, H. Koyama, Y. Nagasaki, K. Kataoka, Polyion complex micelles of pDNA with acetal-poly(ethylene glycol)-poly(2-(dimethylamino)ethyl methacrylate) block copolymer as gene carrier systems: Physicochemical properties of micelles relevant to gene transfection efficacy, Biomacromolecules, 2004, 5 (6), 2128-2136
42)　Y. Haba, A. Harada, T. Takagishi, K. Kono, Rendering poly(amidoamine) or poly(propylenimine) dendrimers temperature sensitive, J. Am. Chem. Soc., 2004, 126 (40), 12760-12761
41)　H. Katakura, A. Harada, K. Kataoka, M. Furusho, F. Tanaka, H. Wada, K. Ikenaka, Improvement of retroviral vectors by coating with poly(ethylene glycol) - poly(L-lysine) block copolymer (PEG-PLL), J. Gene Med., 2004, 6 (4), 471-477
40)　K. Miyata, Y. Kakizawa, N. Nishiyama, A. Harada, Y. Yamasaki, K. Kataoka, H. Koyama, Block Catiomer Polyplexes with Regulated Densities of Charge and Disulfide Crosslinking Directed to Enhanced Gene Expression, J. Am. Chem. Soc., 2004, 126 (8), 2355-2361
39)　D. Wakebayashi, N. Nishiyama, Y. Yamasaki, K. Itaka, N. Kanayama, A. Harada, Y. Nagasaki, K. Kataoka, Lactose-installed polyion complex micelles incorporating plasmid DNA as a targetable gene vector system: Their preparation and gene transfecting efficiency against cultured HepG2 cells, J. Control. Release, 2004, 95 (3), 653-664
38)　M. Jaturanpinyo, A. Harada X. Yuan, K. Kataoka, Preparation of Bionanoreactor Based on Core-Shell Structured Polyion Complex Micelles Entrapping Trypsin in the Core Cross-Linked with Glutaraldehyde, Bioconjuguate Chem., 2004, 15 (2), 344-348
37)　K. Itaka, A. Harada, Y. Yamasaki, K. Nakamura, H. Kawaguchi, K. Kataoka, In situ single cell observation by fluorescence resonance energy transfer reveals fast intra-cytoplasmic deliveryand easy release of plasmid DNA complexed with linear polyethylenimine, J. Gene Med., 2004, 6 (1), 76-84
36)　R. Ideta, Y. Yanagi, Y. Tamaki, F. Tasaka, A. Harada K. Kataoka, Effective accumulation of polyion complex micelle to experimental choroidal neovascularization in rats, FEBS Lett., 2004, 557 (1-3), 21-25

2003

35)　G.-D. Zhang, A. Harada, N. Nishiyama, D.-L. Jiang, H. Koyama, T. Aida, K. Kataoka, Polyion complex micelles entrapping cationic dendrimer porphyrin: Effective photosensitizer for photodynamic therapy of cancer, J. Control. Release, 2003,93 (2), 141-150
34)　A. Harada, K. Kataoka, Switching by Pulse Electric Field of the Elevated Enzymatic Reaction in the Core of Polyion Complex Micelles, J. Am. Chem. Soc., 2003, 125 (50), 15306-15307
33)　K. Itaka, K. Yamauchi, A. Harada, K. Nakamura, H. Kawaguchi, K. Kataoka, Polyion complex micelles from plasmid DNA and poly(ethylene glycol)-poly(L-lysine) block copolymer as serum-tolerable polyplex system: Physicochemical properties of micelles relevant to gene transfection efficiency, Biomaterials, 2003, 24 (24), 4495-4506
32)　Y. Bae, S. Fukushima, A. Harada, K. Kataoka, Design of environment-sensitive supramolecular assemblies for intracellular drug delivery: The polymeric micelles responsive to intracellular pH change, Angew. Chem. Int. Ed., 2003, 42 (38), 4640-4643
31)　A. Matsumoto, S. Ikeda, A. Harada, K. Kataoka, Totally Synthetic Glucose Responsive Polymer Bearing a Novel Phenylborate Derivative as a Glucose-Sensing Moiety Operating at Physiological pH Condition, Biomacromolecules, 2003, 4 (5), 1410-1416
30)　Y. S. Bae, S. Fukushima, A. Harada, K. Kataoka, Preparation and characterization of pH responsive adriamycin-loaded polymeric micelles: Drug release profile and in vitro cytotoxicity on human lung cancer SBC-3, J. Control. Release, 2003, 91 (1-2), 237-239
29)　A. Harada, K. Kataoka, Effect of charged segment length on physicochemical properties of core-shell type polyion complex micelles from block ionomers, Macromolecules, 2003, 36 (13), 4995-5001
28)　G.-D. Zhang, N. Nishiyama, A. Harada, D.-L. Jiang, T. Aida, K. Kataoka, pH-sensitive assembly of light-harvesting dendrimer zinc porphyrin bearing periphery groups of primary amine with poly(ethylene glycol)-b-poly(aspartic acid) in aqueous solution, Macromolecules, 2003, 36 (4), 1304-1309

2002

27)　Y. Yamamoto, K. Yasugi, A. Harada, Y. Nagasaki, K. Kataoka, , Temperature-related change in the properties relevant to drug delivery of poly(ethylene glycol)-poly(D,L-lactide) block copolymer micelles in aqueous milieu, J. Control. Release, 2002, 82 (2-3), 359-371
26)　K. Itaka, A. Harada, K. Nakamura, H. Kawaguchi, K. Kataoka, Evaluation by fluorescence resonance energy transfer of the stability of nonviral gene delivery vectors under physiological conditions, Biomacromolecules, 2002, 3 (4), 841-845
25)　M. Harada-Shiba, K. Yamauchi, A. Harada, I. Takamisawa, K. Shimokado, K. Kataoka, Polyion complex micelles as vectors in gene therapy - pharmacokinetics and in vivo gene transfer, Gene Therapy, 2002, 9 (6), 407-414

2001

24)　Y. Nagasaki, K. Yasugi, Y. Yamamoto, A. Harada, K. Kataoka, Sugar-installed block copolymer micelles: Their preparation and specific interaction with lectin molecules, Biomacromolecules, 2001, 2 (4), 1067-1070
23)　原田敦史・片岡一則，高分子ミセルを基盤とした多機能型遺伝子ベクターの開発，医学のあゆみ，2001, 199 (11-12), 756-760
22)　片岡一則・原田敦史，メディカル・フロンティアに向けてのインテリジェントポリマー設計，高分子，2001, 50 (7), 442-445
21)　Y. Kakizawa, A. Harada, K. Kataoka, Glutathione-sensitive stabilization of block copolymer micelles composed of antisense DNA and thiolated poly(ethylene glycol)-block-poly(L-lysine): A potential carrier for systemic delivery of antisense DNA, Biomacromolecules, 2001, 2 (2), 491-497
20)　A. Harada, K. Kataoka, Pronounced activity of enzymes through the incorporation into the core of polyion complex micelles made from charged block copolymers, J. Control. Release, 2001, 72 (1-3), 85-91
19)　A. Harada, H. Togawa, K. Kataoka, Physicochemical properties and nuclease resistance of antisense-oligodeoxynucleotides entrapped in the core of polyion complex micelles composed of poly(ethylene glycol)-poly(L-lysine) block copolymers, Eur. J. Pharmaceut. Sci., 2001, 13 (1), 35-42
18)　K. Kataoka, A. Harada, Y. Nagasaki, Block copolymer micelles for drug delivery: design, characterization and biological significance, Adv. Drug Delivery Rev., 2001, 47 (1), 113-131
17)　A. Harada, K. Kataoka, Polyion complex micelles with core-shell structure: their physicochemical properties and utilities as functional materials, Macromol. Symp., 2001, 172 (Polymers in Medicine), 1-9

2000

16)　Y. Akiyama, A. Harada, Y. Nagasaki, K. Kataoka, Synthesis of poly(ethylene glycol)-block-poly(ethylenimine) possessing an acetal group at the PEG end, Macromolecules, 2000, 33 (16), 5841-5845
15)　柿澤資訓・原田敦史・片岡一則，環境応答的に解離するアンチセンスDNA内包ポリイオンコンプレックスミセル，インテリジェント材料，2000, 10 (1), 11-13
14)　原田敦史・片岡一則，ウイルス構造に啓発されたドラッグデリバリー用デバイスの創製，蛋白質・核酸・酵素，2000, 45 (7), 1265-1272
13)　S. Cammas-Marion, M.-M. Bear, A. Harada, P. Guerin, K. Kataoka, New macromolecular micelles based on degradable amphiphilic block copolymers of malic acid and malic acid ester, Macromol. Chem. Phys., 2000, 201 (3), 355-364

1999

12)　Y. Kakizawa, A. Harada, K. Kataoka, Environment-sensitive stabilization of core-shell structured polyion complex micelle by reversible cross-linking of the core through disulfide bond, J. Am. Chem. Soc., 1999, 121 (48), 11247-11248
11)　K. Kataoka, A. Harada, D. Wakebayashi, Y. Nagasaki, Polyion complex micelles with reactive aldehyde groups on their surface from plasmid DNA and end-functionalized charged block copolymers, Macromolecules, 1999, 32 (20), 6892-6894
10)　A. Harada, K. Kataoka, On-off control of enzymatic activity synchronizing with reversible formation of supramolecular assembly from enzyme and charged block copolymers, J. Am. Chem. Soc., 1999, 121 (39), 9241-9242
9)　A. Harada, K. Kataoka, Novel polyion complex micelles entrapping enzyme molecules in the core. 2. Characterization of the micelles prepared at nonstoichiometric mixing ratios, Langmuir, 1999, 15 (12), 4208-4212
8)　A. Harada, K. Kataoka, Chain length recognition: Core-shell supramolecular assembly from oppositely charged block copolymers, Science, 1999, 283 (5398), 65-67

1998

7)　K. Kataoka, A. Ishihara, A. Harada, H. Miyazaki, Effect of the secondary structure of poly(L-lysine) segments on the micellization in aqueous milieu of poly(ethylene glycol)-poly(L-lysine) block copolymer partially substituted with a hydrocinnamoyl group at the Ne-position, Macromolecules, 1998, 31 (18), 6071-6076
6)　A. Harada, K. Kataoka, Novel polyion complex micelles entrapping enzyme molecules in the core: Preparation of narrowly-distributed micelles from lysozyme and poly(ethylene glycol)-poly(aspartic acid) block copolymer in aqueous medium, Macromolecules, 1998, 31 (2), 288-294

1997

5)　A. Harada, K. Kataoka, Formation of stable and monodispersive polyion complex micelles in aqueous medium from poly(L-lysine) and poly(ethylene glycol)-poly(aspartic acid) block copolymer, J. Macromol. Sci. Pure. Appl. Chem., 1997, A34 (10), 2119-2133

1996

4)　A. Harada, S. Cammas, K. Kataoka, Stabilized a-helix structure of poly(L-lysine)-block-poly(ethylene glycol) in aqueous medium through supramolecular assembly, Macromolecules, 1996, 29 (19), 6183-6188
3)　S. Cammas, A. Harada, Y. Nagasaki, K. Kataoka, Poly(ethylene oxide-co-b-benzyl L-aspartate) block copolymers: Influence of the poly(ethylene oxide) block on the conformation of the poly(b-benzyl L-aspartate) segment in organic solvents, Macromolecules, 1996, 29 (9), 3227-3231
2)　K. Kataoka, H. Togawa, A. Harada, K. Yasugi, T. Matsumoto, S. Katayose, Spontaneous formation of polyion complex micelles with narrow distribution from antisense oligonucleotide and cationic block copolymer in physiological saline, Macromolecules, 1996, 29 (26), 8556-8557

1995

1)　A. Harada, K. Kataoka, Formation of polyion complex micelles in an aqueous milieu from a pair of oppositely-charged block copolymers with poly(ethylene glycol) segments, Macromolecu

著書

11)　河野健司，原田敦史，「医療応用のための材料化学」, 医療・診断・創薬の化学－医療分野に挑む革新的な科学技術－, 28-35 (2017) 化学同人.
10)　原田敦史, 「酵素と多相系高分子からなる酵素内包コアシェル型ナノ組織体」, コアシェル微粒子の設計・合成技術・応用の展開, pp. 215-223 (2010), シーエムシー．
9)　原田敦史，「バイオコンジュゲートを用いた機能性ゲルの創製」，医療用ゲルの最新技術と開発，pp. 108-114 (2008), シーエムシー．
8)　原田敦史，「ナノバイオデバイスとして機能する高分子ミセル」，次世代医療のための高分子材料工学，pp. 1-10 (2008), シーエムシー．
7)　原田敦史，内核に酵素反応場を有するコア－シェル型ナノ組織体，酵素の活用・利用の最新技術，2006, シーエムシー，第4編第3章
6)　原田敦史，機能化高分子ナノミセル，ソフトナノテクノロジー－バイオマテリアル革命，2005, シーエムシー，230-235
5)　原田敦史・片岡一則，酵素内包コア－シェル型ナノ微粒子の外部刺激による酵素機能制御，ナノバイオエンジニアリングマテリアル，2004, フロンティア出版，209-215
4)　片岡一則・山崎裕一・大塚英典・原田敦史，メディカルナノテクノロジー，ナノテクノロジー基礎シリーズ　バイオナノテクノロジー，2003, オーム社，169-197
3)　原田敦史・片岡一則，高分子ミセルと遺伝子治療，先端化学シリーズ３　糖鎖／バイオマテリアル／分子認識／バイオインフォマティクス，2003, 丸善，198-204
2)　原田敦史・片岡一則，高分子ナノミセルによるDDS，図解ナノテク活用技術のすべて，2002, ㈱工業調査会，282-285
1)　A. Harada, K. Kataoka, Formation of core-shell type nano-associates through electrostatic interaction between peptide and block copolymer with polyethylene glycol segment, Adv. Biomater. Biomed. Eng. Drug Delivery Syst., [5th Iketani Conf. Biomed. Polym.], 1996, 317-318

原田 敦史

学歴

職歴

学位