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Extracellular vesicles as metabolic indicators in disease – in vivo and in vitro studies

演者：Professor Dr. Ewa Stępień （Professor of Institute of Physics, Jagiellonian University, Cracow, Poland）

日時：令和7年5月30日（金）13:30より

場所：中百舌鳥キャンパス A13棟 3階 323室

 The molecular composition of EVs is very complex, with lipid and protein components dominating [1-3]. Although it is known that they are composed of the same macromolecules as the cells from which they originate, we have observed differences between the proportion of macromolecules in different subpopulations of EVs and cells [4]. The proportions and share of individual protein and lipid metabolites also change depending on the metabolic state of the cells from which the individual EV fractions originate [1,5]. One of the main factors influencing their molecular composition is hyperglycemia, which we have observed in an in vitro model of pancreatic beta cells and in clinical studies in patients with diabetes [5-8]. The presentation will show the results of metabolomic studies of small and large EVs isolated from patients with type 1 and 2 diabetes and from in vitro cultures of beta-pancreatic cells in conditions of mild and high hyperglycemia.
References
[1] Marzec ME, et al., Biochem Biophys Res Commun. 2022;622:30-36, [2] Zapała B, Stępień EŁ. Pharm Res. 2023;40(4):817-832, [3] Kasprzyk-Pochopień J, et al., Molecules. 2025;30(6):1384, [3] Stępień EŁ, et al., Biochem Biophys Rep. 2021;25:100888, [4] Roman M, et al., Nanomedicine. 2019;17:137-149, [5] Kamińska A, et al., J Diabetes Res. 2016;2016:5741518, [6] Stępień EŁ, et al., Theranostics. 2018;23;8(14):3874-3890, [7] Kamińska A, et al., Nanomedicine. 2022;39:102468.

（世話人・連絡先：中瀬生彦、藤原大佑、道上雅孝）

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